The group of IGHV1-18 includes 4 alleles, 4 out of the alleles are functional.

For each allele we counted the number of appearances across the population, any appearance was considered valid.

Based on the viewd alleles, we calculated the distance between the germline sequences.

To examine the potential cutoff we observed the sequence depth for each allele

We set an initial cutoff of \(0.5%\) to determine the potential genotype priors. For this cutoff we examined the zygousity state, such as homozygousity, heterozygousity and so on.

With the selected cutoff we saw that there are 3 zygousity states.

From the plots we can that the dominantes alleles 01 and 04. They appear in all states. Alleles 01 and 03 are closer to eachother, hence in state 3 for the threshold of \(0.5%\) I suspect that the sequences are mis-assigned and should be allele 01. Otherwise we should have seen the allele in one of the other stated as well.

Further the relations between 01 and 04 tends to heterozygousity with equal usage, thus the appearance of the lowly expressed allele in state 2 are likely erroneous.

From the results we belive that the cutoff for this group should be closer to \(20%\), and for the adjusted states the allele combinations and the relations are stated in the table below.